Amyloid-β(1-42) alters structure and function of retinal pigmented epithelial cells

Microarray Analysis on Human Neuroblastoma Cells Exposed to Aluminum, β1–42-Amyloid or the β1–42-Amyloid Aluminum Complex

BACKGROUND A typical pathological feature of Alzheimer's disease (AD) is the appearance in the brain of senile plaques made up of β-amyloid (Aβ) and neurofibrillary tangles. AD is also associated with an abnormal accumulation of some metal ions, and we have recently shown that one of these, aluminum (Al), plays a relevant role in affecting Aβ aggregation and neurotoxicity. METHODOLOGY In this...

Blue-light filtering alters angiogenic signaling in human retinal pigmented epithelial cells culture model

BACKGROUND Light exposure and more specifically the spectrum of blue light contribute to the oxidative stress in Age-related macular degeneration (AMD). The purpose of the study was to establish whether blue light filtering could modify proangiogenic signaling produced by retinal pigmented epithelial (RPE) cells under different conditions simulating risk factors for AMD. METHODS Three experim...

Effect of distinct amyloid β1-42 assemblies on synaptic plasticity

mRNA-transfection of Retinal Pigmented Epithelial cells 1 Efficient delivery and functional expression of transfected modified mRNA in human Embryonic Stem Cell-derived Retinal Pigmented Epithelial cells

Geneand cell-based therapies are promising strategies for the treatment of degenerative retinal diseases such as age-related macular degeneration (AMD), Stargardt disease and Retinitis Pigmentosa. Cellular engineering before transplantation may allow the delivery of cellular factors that can promote functional improvements such as increased engraftment or survival of transplanted cells. A curre...

Na,K-ATPase inhibition alters tight junction structure and permeability in human retinal pigment epithelial cells.

Na,K-ATPase regulates a variety of transport functions in epithelial cells. In cultures of human retinal pigment epithelial (RPE) cells, inhibition of Na,K-ATPase by ouabain and K(+) depletion decreased transepithelial electrical resistance (TER) and increased permeability of tight junctions to mannitol and inulin. Electrophysiological studies demonstrated that the decrease in TER was due to an...